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More recently, the freeze-fracture and shadow-casting cryo-SEM (FreSCa cryo-SEM), another single-particle method using a particle-immobilization technique, was developed . Even if a complex machinery is required for this technique, the resolution is pushed down to tens of nanometers. This is the best resolution obtained with “single-particle methods” so far. However, the choice of the oil ...
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Ball milling, [55] cryo-crushing, [56] or high-pressure homogenization [57] are commonly used as physical methods (topdown techniques) to produce cellulose nanofibers from natural sources. For ...